This study provides a description of the systems model, assumptions, and methodologies used in the assessment of the bioenergy potential for the Canadian land adjacent to the Great Lakes St. Lawrence Seaway (GLSLS). It uses a systems approach, where the entire supply chain was modeled by building relationships between the elements that make up the system. The model tracks the flows of mass, energy, and greenhouse gas (GHG) emissions over the lifecycle of the process (Fig. S1). Processes and flows that were beyond the scope of our study are shown outside the system boundary. For example, carbon dioxide (CO2) emissions as a result of biopower generation and green diesel use are outside the system boundary as well as CO2 uptake during plant growth.